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Since only fundamental constants are used for the chromatographic estimation of q, and no adjustable parameters are present, as exist in previous models, this is the best quantitative theory in existence that describes the chromatographic retention of polyelectrolytes on ionexchange columns Its applicability to other biopolymers, such as highly charged nucleic acids, has yet to be determined..

Amazon.com shows us that the Internet isn t a disintermediating technology; it s one that creates new forms of intermediation. Amazon.com is the new channel between publishers and the public, just as ebay.com creates a new intermediation channel for small-scale collectors. Intermediation provides opportunities for creating more reliable and trusted computing models than are possible in the real, two-party world. For example, using an online payment service to transfer funds for an item you bought on eBay eliminates the need to send credit card information to an unknown entity if you trust the payment provider and believe you re going to get the goods (due to eBay feedback ratings), then the three-party system creates more trust than is possible in a two-party system.

W. S. Hancock, Ed., High Performance Liquid Chromatography in Biotechnology, John Wiley & Sons, Inc., New York, 1990. A. Fallon, R. F. G. Booth, and L. D. Bell, Applications of HPLC in Biochemistry, Elsevier, New York, 1987. J.-C. Janson and L. Ryden, Eds., Protein Puri cation: Principles, High-Resolution Methods and Applications, VCH Publishers, New York, 1989. P. R. Haddad and P. E. Jackson, Ion Chromatography: Principles and Applications, Elsevier, New York, 1987. S. Yamamoto, Ion-Exchange Chromatography of Proteins, Marcel Dekker, New York, 1988. I. M. Chaiken, Analytical Af nity Chromatography, CRC Press, Boca Raton, FL, 1987. P. D. G. Dean, W. S. Johnson, and F. A. Middle, Af nity Chromatography: A Practical Approach, Oxford University Press, Washington, DC, 1985. D. J. Winzor, From gel ltration to biosensor technology: the development of chromatography for the characterization of protein interactions. Review article in J. Mol. Recognit. 13, 2000, 279 298.

22

One October afternoon I received a brown-paper package tied up with string, return address somewhere in the Ukraine. At the height of the anthrax mail scares here in New Jersey, I was tempted to microwave the package for several minutes. Despite all of the jokes about My Favorite Things, the contents, in fact, were something I wanted a Russian hockey jersey to add to my growing collection. I found the perfect item on eBay, won the auction, and paid for it using Billpoint, eBay s credit card payment service. The seller received funds in whatever currency she wanted; I was fairly certain I was going to get the jersey because her feedback rating was quite high and she had a perfect record of delivering as promised on jersey auctions. eBay s intermediation allowed me to complete a transaction on the Internet that I never would have done in the real world out of fear of having my credit card number stolen. Hal

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