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Moreover, they include adapters or bridges to databases so that applications functioning in their environment can communicate with the traditional applications of the information system. Finally, in addition to their own adapters, most application servers can embed a broker to ensure the best integration with existing applications. We then obtain the architecture presented in Figure 5.1.

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This kind of interference is called cross-reactivity, or speci c interference, and is usually quantitated by assaying for the interferent in the absence of analyte Validation procedures involve screening of a large number of potential interferents at concentrations higher than their expected levels in real samples Cross-reactivity methods for immunoassays have been described in 6 166 QUALITATIVE (SCREENING) ASSAYS As was stated at the beginning of this chapter, qualitative assays report whether the analyte is present or not in the sample A qualitative yes/no result is important in many clinical assays, where the presence of a certain antibody, for example, indicates a disease state regardless of its concentration Automated instrumental methods, however, provide numerical results, and it is the conversion of these numerical results into a binary yes/no format that is a large component of the validation process Conversion to the binary format requires a cutoff value.

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You re assuming that all clients have nice DNS configurations, and that they ll play by the lifetime and timeout rules, and not make mappings persistent or copy IP addresses into local state files.

I I I I Implicit load balancing doesn t take into consideration the duration of a connection. If every fourth connection is for a rather large file, and all other HTTP hits are for small graphics or HTML files, you ll find that a four-way server farm has a real hotspot where all of the large file transfers cluster. This is an extreme case, but it points to the unpredictability of load balancing via round-robin techniques.

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This value may be selected as the signal corresponding to the LOD or LOQ; it may also be chosen as the signal produced by a legal or accepted cutoff concentration A large sample population is then screened by the new assay and by an accepted (validated) quantitative method Each sample is then characterized as True positive: Both methods yield positive results True negative: Both methods yield negative results False positive: New assay positive, accepted assay negative False negative: New assay negative, accepted assay positive For this categorization, the important assumption has been made that the quantitative method always yields the correct results The predetermined cutoff value is then adjusted to minimize or eliminate false negatives Screening assays are used on large sample populations, often to determine which samples require further investigation For this reason, false positives are not as problematic as false negatives, since they will be further examined.

What started off as a balanced point-counterpoint on DNS round-robin techniques ended up with an extra negative point. This isn t meant to dismiss round-robin DNS out of hand; in some cases it will be the best, quick solution you can implement. However, the Internet market seizes opportunity, and when web server farms became de rigueur, new and established players in IP switching filled the gap in IP traffic distribution.

Network redirection is essentially a reliable DNS round-robin service in a box. An IP redirector assumes a well-known, public hostname and IP address, and then translates it into multiple back-end, private IP addresses. When combined with routing functions, the IP redirector can talk to back ends on multiple networks. Figure 9.9 shows a typical IP redirector configuration, using a redundant IP switch to avoid a single point of failure at the public/network interface. There are several variations on the basic redirector idea, ranging from simple IP address load balancers to stateful, packet-inspecting switches that perform load balancing and failover between back-end targets based on session information.

False negatives, however, can result in misdiagnoses, with severe consequences 1661 Figures of Merit for Qualitative (Screening) Assays Sensitivity percent positives identified True positives 100% True positives false negatives Specificity percent negatives identified True negatives 100% True negatives false positives Efficiency percent correct results True positives True negatives 100% Total number of samples.

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