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This product allowed for a level of availability that had previously been unattainable High Availability for Sun was a tool Like any tool, it could be used well or poorly, depending on the knowledge and experience of the person wielding the tool We implemented several failover pairs that worked very well We also implemented some that worked very poorly The successful implementations were on systems run by experienced and thoughtful SAs who understood the goals of this software, and who realized that it was only a tool and not a panacea The poorly implemented ones were as a result of customers not mirroring their disks, or plugging both systems into the same power strip, or running poor-quality applications, who expected High Availability for Sun to solve all of their system problems automatically.

The people who successfully implemented High Availability for Sun understood that this tool could not run their systems for them They understood that a tremendous amount of administrative discipline was still required to ensure that their systems ran the way they wanted them to They understood that High Availability for Sun was just one piece of the puzzle Today, even though the product once called High Availability for Sun has changed names, companies, and code bases at least three times, there are still people who realistically understand what failover management software (FMS) can and cannot do for them, and others who think it is the be-all and.

decreased respiratory activity by the new method. A false positive involves growth on the agar plate but decreased respiration in the new method. A true negative involves growth on agar, and no charge or an increase in respiration. Finally, a false negative result occurs if there is no observable growth around the lter paper disk by the agar plate method, while the new method shows no charge or an increase in respiratory activity. Table 16.1 summarizes the results obtained in this validation experiment. From the results shown in Table 16.1, three parameters may be calculated: selectivity, sensitivity and ef ciency, as de ned in Eqs. 16.31 16.33. In this example, all three parameters are equal to 100%. Prior to use in a clinical laboratory, this kind of test must undergo regulatory approval. Data are required from several hospital labs, from numerous control and sample organisms, as well as a range of antibiotics. 16.7.2. Measurement of Plasma Homocysteine by Fluorescence Polarization Immunoassay (FPIA) Methodology6 Elevated concentrations of plasma homocysteine (HCY) are related to an increased risk of cardiovascular disease, which exists in numerous forms in plasma, with the main form existing as a disul de with itself, cysteine, or albumin. Therefore, the rst step in the measurement involves treatment with a reducing agent, in this case dithiothreitol (DTT), to obtain HCY in its free form (Eq. 16.34). Some amino acids (e.g., L-cysteine and L-methionine) are present in human plasma at higher molar concentrations than HCY and may interfere with this assay. To avoid this possible interference, the highly selective enzymatic conversion of HCY to S-adenosyl-L-homocysteine (SAH), as shown in Eq. 16.34, is used. Both reactions (reduction and conjugation) are accomplished in 30 min at 34 C.

The component for managing the business rules, used to define the rules for directing the process on the various paths that were modeled This is the component that makes it possible to position these rules outside the code for the applications, and thus to render maintenance and adaptability of the system much simpler and faster..

6

16:34 A monoclonal antibody was raised against SAH, and used in a competitive reaction where the antibody binds either SAH or a uoresceinated SAH analogue (tracer),