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have changed The biggest personal change for me is that my family has had a new addition At this writing, my son Jonathan is almost three years old A more general change over the last 4 years is that computers have become much less expensive and much more pervasive They have also become much easier to use Jonathan often sits down in front of one of our computers, turns it on, logs in, puts in a CD-ROM, and begins to play games, all by himself He can also click his way around Web sites like wwwpbskidsorg I find it quite remarkable that a three-year-old who cannot quite dress himself is so comfortable in front of a computer.

PROBLEMS 1. A mixture consisting of two main protein components was subjected to gel ltration chromatography, with pH 7.1, 0.05 M phosphate as the mobile phase and Sephadex G-100 as the stationary phase. An unretained species, blue dextran, eluted in 2.6 min, while proteins X and Y eluted at times of 6.7 and 9.1 min, respectively. (a) Calculate the relative retention, a, of the two protein components. (b) Which protein has a higher molecular weight 2. A gel- ltration column prepared using Bio-Gel P-100 had a void volume of 5.0 mL and a stationary-phase volume of 50.0 mL. The column was calibrated using six proteins of known molecular weight, by measuring the elution volume of each peak (Table.14.7). An unknown protein was then applied to the column, and its elution volume was measured. Given the data in the table below, determine the molecular weight of the unknown protein.

A Single Window allows participants in the international trading chain to obtain substantial benefits: for governments: - improvement in the collection of taxes; - improvements in regulatory compliance from commercial enterprises; - more efficient and pertinent deployment of resources; for commercial enterprises: - reduction in costs through a reduction in delays; - greater rapidity in customs clearance and notices; - explanations and predictable applications of regulations; - more efficient and pertinent deployment of resources. For example, according to studies from UN/CEFACT, when the Republic of Mauritius put a Single Window in place, the average time for customs clearance for undisputed declarations of merchandise went from four hours to 15 minutes. In the same way, the mechanism of the Single Window in Senegal was able to reduce to one day the administrative processes that previously took two or three days. The costs of implementing a Single Window vary depending on the approach: for example, in the case of a Window financed by governmental funds, these costs are most often integrated in the commercial development policy budget for the country. Different implementation models exist for a Single Window, depending on the requirements and local or regional conditions: financing can come from the government (as in Finland, Sweden or the USA), the private sector (as in Guatemala or Germany), or via mixed private/public initiatives (as in China, Republic of Mauritius, Senegal, Malaysia or Singapore); the use of the Single Window can be mandatory (as in Finland, the Republic of Mauritius, Senegal or Guatemala), or based on voluntary participation (as in China, Germany, Malaysia, Sweden or the USA); services are variable, and can be free (as in Finland, Sweden or the USA), or use various billing schemes (as in Guatemala, Germany, China, Malaysia, Republic of Mauritius, Senegal, and Singapore).

The biggest societal change that has taken place in the last 4 years (and, in fact, in much longer than the last 4 years) occurred on September 11, 2001, with the terrorist attacks on New York and Washington, DC I am a lifelong resident of the New York City suburbs, in northern New Jersey, where the loss of our friends, neighbors, and safety is keenly felt by everyone But for the purposes of this book, I will confine the discussion to how computer technology and high availability were affected In the first edition, we devoted a single chapter to the subject of disaster recovery, and in it we barely addressed many of the most important issues.

In this, the second edition, we have totally rewritten the chapter on disaster recovery ( 20, A Disaster Recovery Plan ), based in part on many of the lessons that we learned and heard about in the wake of September 11 We have also added a chapter ( 21, A Resilient Enterprise ) that tells the most remarkable story of the New York Board of Trade, and how they were able to recover their operations on September 11 and were ready to resume trading less than 12 hours after the attacks When you read the New York Board of Trade s story, you may notice that we did not discuss the technology that they used to make their recovery.