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Even when the assay has been optimized in theory, practical work requires care and technical skill for optimum performance Completeness of recovery in each step should be checked independently, for example, by using an independent tracer to follow the progress of a reaction Equilibration rates may be very sensitive to temperature, the order of addition of reagents and stir rates Assaying standards in each run exactly the same way as samples counterbalances some minor errors; however, poor timing or temperature control often results in increased coef cients of variation for replicate measurements Incubation times (when necessary) are often shortened to the minimum, and room temperature incubations are common; if equilibration has not occurred, minor technical variations may have exaggerated effects (eg, room temperature may vary considerably from summer to winter).

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We take a very layered approach to systems, and that layered approach follows in the organization of this book. We look at each type of component separately, starting with disks and moving up through systems into software and finally disaster recovery. In many of the chapters, you will find sidebars that we call Tales from the Field. They are generally true-to-life experiences that we have lived through ourselves or have seen real users of computer systems go through. Some of them are funny; all of them help make a point clearer. We hope that you learn from the mistakes and examples that we have seen and sometimes suffered through. 2, What to Measure, takes a look at the numbers and statistics that go into measuring availability, as well as what can go wrong with the various components of your system. This chapter also looks at measurement trends, such as six sigma and nines, that are becoming more common metrics for system quality. 3 introduces the Availability Index in some detail and looks at the business case for availability. 4, The Politics of Availability, is a primer on how to introduce the concept of availability into your organization and how to deal with some of the politics and other potential organizational roadblocks.

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5, 20 Key High Availability Design Principles, is a cornerstone for the whole book and contains our 20 key design principles for attaining resilience and high availability We refer back to these principles throughout the book 6, Backups and Restores, focuses on the last line of defense between you and irrevocably lost data How do you take backups, why do you take them, and how do you handle the tapes and the hardware 7, Highly Available Data Management, looks at data storage We discuss disks and disk arrays, and the ways to logically arrange them to maximize your protection against hardware failures, including the use of RAID (redundant array of independent disks) hardware and software 8, SANs, NAS, and Virtualization, introduces some newer technology: Storage Area Networks (SANs), Network-Attached Storage (NAS), and virtualization.

Decisions must be made about the method based on the quality of the data produced, and quality criteria must be established This task is not trivial, since new guidance documents are continually produced to determine whether an analytical.

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9, Networking, reviews networking concepts and discusses the various elements of making your networks survive failures at the physical or logical layers, including router redundancy and load balancing 10, Data Centers and the Local Environment, looks at the environment around your computers, including your data center, power issues, cabling, maintenance plans, cooling, system naming conventions, and physical space 11, People and Processes, looks at some people and process issues related to system resiliency, such as system administrators, maintenance plans, patches, documentation, vendor management, and security 12, Clients and Consumers, examines clients as consumers of high-availability services and as resources to be managed 13, Application Design, focuses on application reliability.

Bianalytical Chemistry, by Susan R. Mikkelsen and Eduardo Corton ISBN 0-471-54447-7 Copyright # 2004 John Wiley & Sons, Inc.

What are the issues involved in making your applications recover properly after a server has failed and recovered What should you do to make your applications more resilient to external system faults and internal (logical) problems 14, Data and Web Services, details high-availability models for web and network services such as web, application, and directory servers, along with some thoughts on making XML-based web services more reliable 15, Local Clustering and Failover, introduces the concept of clustering, the technology that is often viewed as the core of high availability In 16, Failover Management and Issues, we look at failovers and the software that manages them We look at the implications of building your own failover software, and at some of the inherent complexities that accompany the use of this software 17, Failover Configurations, examines the myriad of different failover configurations the good, the bad, and the ugly.

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