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2. In which case (low or high MW) is the monoisotopic peak intensity higher than isotopic peak intensity 3. A protein molecule is protonated using an ESI nanoelectrospray system, and analyzed using a mass lter (quadrupole). The Mr (average mass) of this protein is 23,630.36. Use adequate nomenclature to symbolize the molecular ion family, for the charge range 1 to 6. Calculate the mass of each ion, and its position in the mass spectrum obtained. 4. b-Lactoglobulin was puri ed, and an ESI spectrum was obtained, as shown in the Figure 15.16. Calculate the protein mass for each peak and the average for all the peaks. Present a hypothesis to explain the absence of low-charged species.

Another way of making the point about balancing downtime with cost is to look at availability as a curve. The more you spend, the higher you move up the curve. Be sure to note, however, that the incremental costs to move from one level to the next increase as you move up the curve. As you go through the technology chapters, which make up the heart of the book, you ll find that each chapter begins with a picture of the Availability Index. A detailed explanation can be found in 3, The Value of Availability, but fundamentally, it shows the relationship between availability and cost. The line is curved because the relationship is not linear; the higher the level of availability you require, the more it costs to move to the next level. Costs accelerate as you move up the Index. Another way to look at it is that the curve reflects the law of diminishing returns. After you protect against the

5. Calculate the resolution for the following mass spectrum (Fig. 15.17).

1

cheaper and easier-to-fix problems, the cost (and complexity) of the next level of protection increases, flattening out the curve. You may also notice that the curve never quite makes it to 100 percent; that s because we believe that 100 percent availability cannot be attained over the medium and long term. More on that later.

161 INTRODUCTION Validation has the primary goal of selecting the best available method for detection and/or quantitation, based on objective performance data Regulations require that clinical laboratories show documentation and validation data when new tests are introduced These data are also important for quality control and troubleshooting The successful use of an assay depends rst on a fundamental understanding of the analytical principles governing the assay Assay characteristics such as precision, accuracy, and detection limit depend on the properties of the components, the assay format, and the detection system For example, both manual and automated assays may have problems associated with timing or reaction rates, but the automated method may be expected to have better precision in reagent delivery Both may have imprecision as a result of low analyte concentration or a slow approach to equilibrium.

Summary

Computer systems are very complex. The solutions that make them work best are complex too. They require planning, work, testing, and a general ongoing effort by knowledgeable people at every level of implementation. Applications must be tolerant of failures and inappropriate inputs. Hardware must be able to automatically recover from failures. And since systems change, if you want this functionality to always be present, you must regularly test your recovery procedures. Do they still work Do they still work as quickly as they should If they do not work as expected, the time to find out is not when something has gone wrong and the pressure is on. Our goals are to help you design your systems for maximum availability and predictable downtime and recovery times. We will look at these systems from end to end, and from network, system, and application perspectives.

4.5.2. Elements of an SOA infrastructure Advocating the de-correlation of the call from the application to the SOA service directly: a services infrastructure must be used . Even if, today, no specific definition exists for what a services infrastructure must contain, it is still possible to list its principal functions: Multi-channel communication: to ensure dependable, high-performance communication with existing applications, such as with the new Web applications, the infrastructure must not be limited only to SOAP implementations, but offer different communication channels (such as message exchanges by queue managers). Mediation/transformation: these functionalities are necessary to make the deployment flexible in a distributed context, in particular with an existing application base. Mediation/transformation makes it possible to adjust the constraints between consumers and producers in, for example, data formats or type of communication used. It should be noted that these functions, which are at the core of EAI tools, are also the basis of service infrastructures. Services registry: a registry of services is an indispensable function for offering the functionalities of dynamic services discovery, and for responding to the need to