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where A represents the activator species. Parallel reaction sequences must be considered if the enzyme is catalytically active in the absence of activator. With the exception of prosthetic group activation, activators are not usually speci c, and several species may have similar activating effects on an enzyme. For example, isocitrate dehydrogenase is activated by both Mn2 and Mg2 ; it has been shown that, in the absence of Mg2 , Mn2 levels as low as 5 ppb may be determined via measurement of isocitrate dehydrogenase activity. Anions are also relatively nonspeci c activators, and the activation of a-amylase by buffer anions (especially Cl ) has been studied in detail. An exception to this nonspeci c ion activation occurs with pyruvate kinase: This enzyme is activated by K , but is inhibited by Na . While activator concentrations may be quantitated through activity assays, the presence of unknown concentrations of activators in samples requiring substrate or enzyme quantitation represents a signi cant source of error. If activators are present or suspected in such samples, activators are added in excess to both samples and calibration standards.
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4.5.1.4. Reuse The reuse of existing components is a fundamental characteristic of services architectures. It is through reuse that agility and reactivity are conferred on the information system, by accelerated development of new applications that will use these components. To achieve this, callable components must be carefully selected and defined as services that can be used by a large number of clients. Technological neutrality and the exposition of services in a registry will facilitate this reuse, similar to loose coupling, discussed next. 4.5.1.5. Loose coupling One of the principal characteristics of the SOA approach lies in how a service is organized with its consumers. Specifically, services are designed without regard for any particular service consumer. Inside the service, no assumption is made concerning the objective or nature technical or functional of the service consumer. In this way, a service is totally decoupled from the service consumer. On the other hand, the consumer is dependent on the service, since it refers to the service interface. As a result, the SOA approach allows the implementation of an architecture with loose coupling. It is thus differentiated from a monolithic architecture, in which all the software components are designed to function in the initially projected context (known as strong coupling logic).
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Consolidation is, however, a powerful force for improving the simplicity and manageability of an environment. It comes with the cost of having to invest even more engineering effort in making the larger, consolidated server more reliable and robust. The moral of the story is Go ahead and put all your eggs in one basket; just make sure the basket is built out of titanium-reinforced concrete. A new trend is emerging, though, that may render this particular principle moot in a few years. Vendors are linking together hundreds or even thousands of very small systems called blades, and developing pseudo-backplanes and shared networks to connect them together, enabling these blade computers to act as a massive cluster. The biggest obstacle so far is administration; few mainstream tools allow easy administration of hundreds of systems, even ones that share applications and operating systems. Those technologies will likely appear in the next few years. We ll discuss blade technology further in 22, A Brief Look Ahead.
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1 R A W Johnstone and M E Rose, Mass Spectrometry for Chemists and Biochemists, Cambridge University Press, New York, 1996, pp 34 41 2 G Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp 9 17 3 G Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp 11 18 4 R A W Johnstone and M E Rose, Mass Spectrometry for Chemists and Biochemists, Cambridge University Press, New York, 1996, pp 34, 105 109 5 C N McEwen and B S Larsen, in Mass Spectrometry of Biological Materials, B S Larsen and C N McEwen, Eds, Marcel Dekker, New York, 1998, pp 10 22 6 C N McEwen and B S Larsen, in Mass Spectrometry of Biological Materials, B S Larsen and C N McEwen, Eds, Marcel Dekker, New York, 1998, pp 17 19 7 G.
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