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The cells to be analyzed (mainly bacteria, but fungus or virus particles have also been studied) are typically physically or chemically lysed; exposure to the matrix solution may be suf cient to lyse the cells Spores are digested (eg, with strong organic acids), and then the MALDI matrix is added Interpretation of bacterial mass spectra is done with dif culty First, a large portion of cellular compounds are common between different bacterial strains Second, the spectral reproducibility for a given bacterial strain and protocol/instrument is poor, whether it is tested in the same or different laboratories There are a number of biological and instrumental sources of variability, including differences in cellular composition for different culture phases, culture media, and stress; minor variations in the sample/matrix ratio cause signi cant modi cation in the resulting spectra Figure 1515 shows typical bacterial spectra obtained for one strain of E.

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In 3, The Value of Availability, we introduced the idea of the Availability Index. As shown in Figure 5.1, the first level of the Index is called Good System Administration Practices. Since these practices are the first level of the Index, they are the least expensive to implement. Being on the first level doesn t make them the easiest, it just means that they lay the foundation for all of the other technologies that we will discuss in future chapters. We believe that there is no good reason to skip over these most basic system administrative practices. Some of the practices that we ll discuss in this chapter are easy to implement, while others can be difficult and tedious. The difficult ones are no less important than the easy ones. Most, though not all, of these topics will only be discussed here. Later sections of the book discuss technologies and principles of availability that rank higher on the Index, and therefore are more expensive and complex. For no particular reason, we present this list in reverse order, from #20 down to #1.

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5

coli; note that many peaks are common to both spectra Efforts are underway to improve reproducibility, and good results have been obtained by some authors33 Two main approaches have been used to interpret bacterial mass spectra First, pattern recognition methods have been used, in which reference spectra are obtained under standardized conditions to generate a ngerprint collection An unknown bacterial spectrum obtained under the same conditions is then compared to the collection and matched if possible A second approach exploits the information contained in genome and protein sequencing databases The masses of a set of proteins from the bacterial strain under study are determined and used to search databases for protein molecular mass matching This has been used as a method to differentiate B subtilis and E coli, two organisms that have completely sequenced genomes34 SUGGESTED REFERENCES.

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451 Characteristics of an SOA Even if no unanimously accepted definition exists for service-oriented architecture, it is still possible to determine the characteristics that every observer accepts 4511 The service interface: the heart of the SOA concept A service is an operational encapsulated software component presented as a pair of separately defined elements: the interface for the service and the implementation of the service (Figure 417) The interface is the fundamental component of a service A service is always intended for access via programming from another component (a service consumer) The service interface defines the contract of access through service programming: it establishes the identity of the service and the rules for calling the service The service interface must have enough information to identify the service and use it without taking its internal design or its implementation content into consideration.

G. Siuzdak. Mass Spectrometry for Biotechnology, Academic Press, New York, 1996. B. S. Larsen and C. N. McEwen, Eds., Mass Spectrometry of Biological Materials, Marcel Dekker, New York, 1998.

One of the basic rules of life in the 21st century is that quality costs money. Whether you are buying ice cream ( Do I want the Ben & Jerry s at $4.00 per pint, or the store brand with the little ice crystals in it for 79 cents a gallon ), cars (Rolls-Royce or Saturn), or barbecue grills, the higher the quality, the more it costs. As we explained in 3, the decision to implement availability is a business decision. It comes down to dollars and cents. If you look at the business decision to implement availability purely as how much will it cost me, then you are missing half of the equation, and no solution will appear adequate. Instead, the decision must also consider how much the solution will save the business and balance the savings against its cost. When considering the return on investment of a particular availability solution, look at how it will increase uptime and increase the value of your systems because they are up more. Consider the increase in productivity among the users of the critical system and how, because the system is up, they won t have to work overtime or be idled during the workweek. There is no question that

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