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13.3.2. Fixed-Angle Rotors In this rotor the sample tubes t into holes in a solid rotor. The tube angle therefore remains constant during the loading, centrifugation, and unloading process. When the centrifugation force increases, the solution reorients inside the tube. For a given sample volume, the centrifugation path with a xed-angle rotor is shorter than that with a swinging-bucket rotor. The particles reach the tube wall more rapidly, and then slide down the wall to form a pellet (Fig. 13.2). These rotors are more ef cient for differential pelleting or other pellet-based procedures, and could be used for isopycnic experiments, which are equilibrium density gradient techniques, described later in this chapter. When the rotor angle is shallow, the sedimentation path is shorter, and the pelleting ef ciency is increased. Fixed angle rotors are available with angles between 14 and 40 . 13.3.3. Vertical Rotors In vertical rotors (Fig. 13.2) the tubes are xed in a vertical position during centrifugation, and when the centrifugal force increases the solution reorients to the g force. The reorientation process is explained later in this chapter. For now, this means that in this rotor the tube covers are important, and must be able to withstand

8

CLEARING FACTOR (k )

For applications where many servers will be sharing large amounts of data, SAN is better SAN is better for large backups and for high-speed file sharing, where speed and bandwidth are the primary concerns In a SAN, hosts and storage communicate directly In a NAS environment, the client does not communicate directly with the storage Instead, requests go through another computer, a server The server processes the request with data on its local disks and sends data back to the client via a multilayered protocol (NFS running on top of TCP/IP, running on an Ethernet, for example) As a result, given similar network hardware, SAN tends to run faster than NAS, although it is very difficult to make absolute statements about performance If you are concerned about performance, be sure to run benchmarks that match your local environment as closely as possible.

In a NAS, servers deliver filesystems to their clients Any mirroring or other volume management is performed at a lower level, and the clients do not see it This greatly reduces the administrative overhead of NAS on clients In a SAN, on the other hand, hosts on the SAN see disk blocks Any work to combine or arrange the disk blocks must be done on each client If SAN-based hosts attempt to write to the shared disks when or where they should not, the result can be data corruption, as the different hosts get confused about what is on the disks Building a SAN can be a very complex endeavor Many different components from many different vendors are required, and not all combinations are compatible, or have even been tested together.

a large hydrostatic pressure. The sedimentation path is the shortest of all kinds of rotors described here (the tube diameter), and the minimum g force, related to the minimal radius, is larger for this rotor, allowing faster separations at lower speeds. These rotors are the rst choice for isopycnic centrifugation, can be used for ratezonal centrifugation, and are never used for pellet-related techniques, because the pellet is formed over the entire tube wall, and usually detaches easily. 13.4. CLEARING FACTOR (k ) Pelleting capacity is related to the value of the clearing factor k, a variable that refers to the ability of a set of centrifugation conditions to separate or pellet particles. Smaller k values indicate greater ef ciency. The parameter k is de ned as follows:6 k t s 1013 13:6

It is very likely that SANs will become much easier to build and administer over the coming years, but they have a very long way to go before they are as easy to manage as a filer, which, as we said earlier, is little more than a black box with few, if any, buttons and knobs NAS tends to be a much less expensive solution to deploy than SANs, since it employs older and more standardized technology While a single HBA for a SAN can cost around $1,000 (a number that is sure to drop significantly over time), a 100Mbit Ethernet card can cost less than $20 In a large network with lots of hosts, the cost savings for NAS will be quite significant But the performance with NAS can be significantly slower.